- Human(2)
Recombinant Human NFE4 Protein, Myc/DDK-tagged, C13 and N15-labeled
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Cat.No. : | NFE4-1532H |
Product Overview : | NFE4 MS Standard C13 and N15-labeled recombinant protein (NP_001078855) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
Description : | The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. |
Source : | HEK293 |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 20.33 kDa |
AA Sequence : | LDPVPRRSAA AIALPRVVCW HTLKSLNGYK NLSSGAETRE GLRSSSPVDL PLRPRKQATA AGQRKLLSLQ LLLCACTSVT DLTYWGPAGH GATAPHRSLL AIHLHLVPAS SAAMKATGPH NAQTQVNPRG HAPSAEDPTG SWTVSGPCKD HPHPFLSQSN PPTRISSALP LKTDSALEQT PQQLPSLHLS QGTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : | 50 μg/mL as determined by BCA |
Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
SDS-PAGE : |
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Gene Name : | NFE4 nuclear factor, erythroid 4 [ Homo sapiens (human) ] |
Official Symbol : | NFE4 |
Synonyms : | NFE4; nuclear factor, erythroid 4; NF-E4; fetal globin activator NF-E4; gamma-globin gene activator; transcription factor NF-E4 |
Gene ID : | 58160 |
mRNA Refseq : | NM_001085386 |
Protein Refseq : | NP_001078855 |
MIM : | 612133 |
UniProt ID : | Q86UQ8 |
◆ Recombinant Protein
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