Recombinant Human NFKB1 protein, His & GST-tagged

• Cat.No.: NFKB1-1847H
• Product Overview: Recombinant Human NFKB1 aa. (Gln805~Ser892 (Accession # P19838)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.1kDa.
• Protein length: Gln805~Ser892 (Accession # P19838)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NFKB1 nuclear factor kappa B subunit 1 [ Homo sapiens (human) ]
• Official Symbol: NFKB1
• Synonyms: TNNT1; troponin T1, slow skeletal type; ANM; TNT; NEM5; STNT; TNTS; troponin T, slow skeletal muscle; slow skeletal muscle troponin T; troponin T type 1 (skeletal, slow); troponin-T1, skeletal, slow
• Gene ID: 4790
• mRNA Refseq: NM_001165412.1
• Protein Refseq: NP_001158884.1
• UniProt ID: P19838

Reviews

06/16/2022

Potent biological activity of NFKB1, combined with its high purity, provides accurate and reliable experimental data for the research field.

04/22/2022

The short half-life and high clearance of NFKB1 allow its rapid action and rapid clearance in vivo, improving therapeutic efficiency.

05/21/2019

Beacuse production process of NFKB1 realizes the minimization of waste and the minimal impact on the environment, which is truly green production.

Q&As

What is the role of NFKB1 protein in autoimmune diseases?

NFKB1 protein is involved in the occurrence and progression of autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus.

What is the relationship between NFKB1 protein and apoptosis?

NFKB1 protein plays a complex role in apoptosis and may promote or inhibit the occurrence of apoptosis.

What drugs interact with the NFKB1 protein?

A variety of drugs have been developed that target the NFKB1 protein and its downstream signaling pathways, such as inhibiting NFKB1 activation or inhibiting the transcriptional activity of NFKB1 downstream genes.

Does the NFKB1 protein study have implications for new drug development?

The protein plays a key role in immune regulation and inflammatory response, and studying the function and regulatory mechanism of NFKB1 protein will help the development of new drugs, such as targeted drugs targeting NFKB1 protein and its signaling pathway may be applied to the treatment of related diseases.

Is there a potential application of NFKB1 protein in the design of immune vaccines?

This protein plays an important role in the activation of immune cells and the regulation of inflammation, so it can be used in immune vaccine design to enhance immune effects.

Why does NFKB1 protein have a pattern of expression in immune cells?

The expression pattern of NFKB1 protein in immune cells is regulated by different stimuli and environments, and participates in the activation and regulation of immune cells.