Recombinant Human NFYA Protein, His-tagged

• Cat.No.: NFYA-200H
• Product Overview: Recombinant Human NFYA, transcript variant 1, fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl
• Molecular Mass: 36.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: NFYA nuclear transcription factor Y, alpha [ Homo sapiens ]
• Official Symbol: NFYA
• Synonyms: NFYA; nuclear transcription factor Y, alpha; nuclear transcription factor Y subunit alpha; CBF B; HAP2; NF YA; HAP2 CCAAT-binding protein; Transcription factor NF-Y, A subunit; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; nuclear transcription factor Y subunit A; CCAAT-binding transcription factor subunit B; CBF-A; CBF-B; NF-YA; FLJ11236
• Gene ID: 4800
• mRNA Refseq: NM_002505
• Protein Refseq: NP_002496
• MIM: 189903
• UniProt ID: P23511