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Recombinant Human NNMT Protein, His-tagged, Bioactivity Validated

Cat.No.: NNMT-234H
Product Overview: Recombinant Human NNMT(1-264aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.
Description: NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Source: E. coli
Species: Human
Tag : His
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Bio-activity: Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Molecular Mass: 37.7 kDa (284aa), confirmed by MALDI-TOF
Protein length: 1-264aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ
LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV
LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE
GLFSLVARKL SRPL
Purity: > 95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 1mg/ml (determined by Absorbance at 280nm)
Gene Name: NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
Official Symbol: NNMT
Synonyms: NNMT; nicotinamide N-methyltransferase;
Gene ID: 4837
mRNA Refseq: NM_006169
Protein Refseq: NP_006160
MIM: 600008
UniProt ID: P40261

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