Recombinant Human NONO Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NONO-1688H
• Product Overview: NONO MS Standard C13 and N15-labeled recombinant protein (NP_001138881) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 54.2 kDa
• AA Sequence: MQSNKTFNLEKQNHTPRKHHQHHHQ QQHHQQQQQQPPPPPIPANGQQASS QNEGLTIDLKNFRKPGEKTFTQRSR LFVGNLPPDITEEEMRKLFEKYGKA GEVFIHKDKGFGFIRLETRTLAEIA KVELDNMPLRGKQLRVRFACHSASL TVRNLPQYVSNELLEEAFSVFGQVE RAVVIVDDRGRPSGKGIVEFSGKPA ARKALDRCSEGSFLLTTFPRPVTVE PMDQLDDEEGLPEKLVIKNQQFHKE REQPPRFAQPGSFEYEYAMRWKALI EMEKQQQDQVDRNIKEAREKLEMEM EAARHEHQVMLMRQDLMRRQEELRR MEELHNQEVQKRKQLELRQEEERRR REEEMRRQQEEMMRRQQEGFKGTFP DAREQEIRMGQMAMGGAMGINNRGA MPPAPVPAGTPAPPGPATMMPDGTL GLTPPTTERFGQAATMEGIGAIGGT PPAFNRAAPGAEFAPNKRRRYTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NONO non-POU domain containing octamer binding [ Homo sapiens (human) ]
• Official Symbol: NONO
• Synonyms: NONO; non-POU domain containing, octamer-binding; non POU domain containing, octamer binding; non-POU domain-containing octamer-binding protein; NMT55; non Pou domain containing octamer (ATGCAAAT) binding protein; NRB54; Nuclear RNA binding protein; 54 kD; P54; P54NRB; p54(nrb); 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex, 52 kDa subunit; non-POU domain-containing octamer (ATGCAAAT) binding protein
• Gene ID: 4841
• mRNA Refseq: NM_001145409
• Protein Refseq: NP_001138881
• MIM: 300084
• UniProt ID: Q15233