Recombinant Human NOS3 protein, His-tagged

• Cat.No.: NOS3-7856H
• Product Overview: Recombinant Human NOS3 aa. (Arg756~Gln888 (Accession # P29474)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Arg756~Gln888
• Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NOS3 nitric oxide synthase 3 [ Homo sapiens (human) ]
• Official Symbol: NOS3
• Synonyms: NOS3; nitric oxide synthase 3; eNOS; ECNOS; nitric oxide synthase, endothelial; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; endothelial NOS; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21
• Gene ID: 4846
• mRNA Refseq: NM_000603.4
• Protein Refseq: NP_000594.2
• UniProt ID: P29474