Recombinant Human NPHS1 Protein, His&GST-tagged

• Cat.No.: NPHS1-1843H
• Product Overview: Recombinant Human NPHS1 Protein (Arg1160-Val1241) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 39.4 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Arg1160-Val1241
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: NPHS1 NPHS1 adhesion molecule, nephrin [ Homo sapiens (human) ]
• Official Symbol: NPHS1
• Synonyms: NPHS1; NPHS1 adhesion molecule, nephrin; CNF; NPHN; nephrin; nephrin; NPHS1, nephrin; nephrosis 1, congenital, Finnish type (nephrin); renal glomerulus-specific cell adhesion receptor; truncated NPHS1
• Gene ID: 4868
• mRNA Refseq: NM_004646
• Protein Refseq: NP_004637
• UniProt ID: O60500