Active Recombinant Human NQO1 protein, His-tagged

• Cat.No.: NQO1-138H
• Product Overview: Active Recombinant Human NQO1 protein(P15559)(2-274 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-274 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: NAD(P)H: quinone acceptor oxidoreductase 1 (NQO1), also known as DT-diaphorase, is a widely-distributed FAD-dependent flavoprotein that promotes 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes. As a result it prevents the one electron reduction of quinones that results in the production of radical species. NQO1 is a highly-inducible enzyme that is regulated by the Keap1/Nrf2/ARE pathway. The increase and decrease of NQO1 levels are associated with decreased and increased susceptibilities to oxidative stress, respectively. Thus, NQO1 is a marker cytoprotective enzyme in oxidative stress. Independently of its catalytic function, NQO1 plays a role in regulating the proteosomal degradation of p53, p73a, and p33. NQO1 physically interacts with p53 and p73 in an NADH-dependent manner and protects them from 20S proteasomal degradation in a ubiquitin independent pathway. The activity assay of recombinant human NQO1 was measured by its ability to oxidize the substrate resazurin to resorufin. The rhNQO1 was diluted to 100 µg/ml in the assay buffer 50 mM HEPES, 0.2 M NaCl, 5 µM FAD, 0.05% Tween 20, pH 7.5. 50 µl 100 µg/ml rhNQO1 was added into the microplate and start the reaction by adding 50 µl substrate mixture of 400 uM beta-NADH and 20 uM resazurin which was diluted in assay buffer. Read at excitation and emission wavelengths of 540 nm and 585 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human NQO1 is >18 pmol/min/µg.
• Molecular Mass: 34.4kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NQO1 NAD(P)H dehydrogenase, quinone 1 [ Homo sapiens ]
• Official Symbol: NQO1
• Synonyms: NQO1; NAD(P)H dehydrogenase, quinone 1; DIA4, diaphorase (NADH/NADPH) (cytochrome b 5 reductase) , NMOR1; NAD(P)H dehydrogenase [quinone] 1; DHQU; DTD; QR1; azoreductase; diaphorase-4; DT-diaphorase; dioxin-inducible 1; menadione reductase; quinone reductase 1; phylloquinone reductase; NAD(P)H:quinone oxireductase; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); DIA4; NMOR1; NMORI
• Gene ID: 1728
• mRNA Refseq: NM_000903
• Protein Refseq: NP_000894
• UniProt ID: P15559