Recombinant Human NRAS Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NRAS-3519H
• Product Overview: NRAS MS Standard C13 and N15-labeled recombinant protein (NP_002515) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 21.2 kDa
• AA Sequence: MTEYKLVVVGAGGVGKSALTIQLIQ NHFVDEYDPTIEDSYRKQVVIDGET CLLDILDTAGQEEYSAMRDQYMRTG EGFLCVFAINNSKSFADINLYREQI KRVKDSDDVPMVLVGNKCDLPTRTV DTKQAHELAKSYGIPFIETSAKTRQ GVEDAFYTLVREIRQYRMKKLNSSD DGTQGCMGLPCVVMTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NRAS NRAS proto-oncogene, GTPase [ Homo sapiens (human) ]
• Official Symbol: NRAS
• Synonyms: NRAS; neuroblastoma RAS viral (v-ras) oncogene homolog; GTPase NRas; N ras; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; NS6; ALPS4; N-ras; NRAS1
• Gene ID: 4893
• mRNA Refseq: NM_002524
• Protein Refseq: NP_002515
• MIM: 164790
• UniProt ID: P01111