Active Recombinant Human NRG1 protein

Species :	Human
Source :	E.coli
Tag :	Non
Protein Length :	177-241 aa
Form :	Lyophilized from a 0.2 μm filtered solution in 1 × PBS, pH 7.4, with 5 % trehalose.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 0.5 ng/ml, corresponding to a specific activity of ＞ 2.0 × 10^6IU/mg.
Molecular Mass :	Approximately 7.5 kDa, a single non-glycosylated polypeptide chain containing 65 amino acids.
AASequence :	SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
Endotoxin :	Less than 0.1 EU/μg of rHuNRG1-β1, 177-241a.a. as determined by LAL method.
Purity :	> 97 % by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	NRG1 neuregulin 1 [ Homo sapiens ]
Official Symbol	NRG1
Synonyms	NRG1; neuregulin 1; HGL; pro-neuregulin-1, membrane-bound isoform; GGF; HRG; NDF; MSTP131; pro-NRG1; glial growth factor; neu differentiation factor; neuregulin 1 type IV beta 3; neuregulin 1 type IV beta 1a; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); ARIA; GGF2; HRG1; HRGA; SMDF; MST131;
Gene ID	3084
mRNA Refseq	NM_001159995
Protein Refseq	NP_001153467
MIM	142445
UniProt ID	Q02297