|Product Overview :||Biotinylated Human NRP1, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # O14786-1(V179A)). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.|
|Description :||Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.|
|Tag :||His & Avi|
|Predicted N Terminal :||Phe 22|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 73.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Phe 22 - Lys 644|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
Biotinylated Human NRP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||NRP1 neuropilin 1 [ Homo sapiens (human) ]|
|Official Symbol :||NRP1|
|Synonyms :||NP1; NRP; BDCA4; CD304; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; neuropilin-1|
|Gene ID :||8829|
|mRNA Refseq :||NM_001024628.2|
|Protein Refseq :||NP_001019799.1|
|UniProt ID :||O14786|