Recombinant Human NT5C protein, His & T7-tagged

• Cat.No.: NT5C-1074H
• Product Overview: Recombinant Human NT5C aa. (Asp12~Leu200 fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.5kDa.
• Protein length: Asp12~Leu200
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: NT5C 5', 3'-nucleotidase, cytosolic [ Homo sapiens (human) ]
• Official Symbol: NT5C
• Synonyms: NT5C; 5', 3'-nucleotidase, cytosolic; DNT; cdN; DNT1; P5N2; PN-I; HEL74; PN-II; UMPH2; dNT-1; 5'(3')-deoxyribonucleotidase, cytosolic type; 5' nucleotidase, deoxy (pyrimidine), cytosolic type C; cytosolic 5',3'-pyrimidine nucleotidase; deoxy-5'-nucleotidase 1; epididymis luminal protein 74; uridine 5'-monophosphate phosphohydrolase 2; uridine 5-prime monophosphate hydrolase 2
• Gene ID: 30833
• mRNA Refseq: NM_001252377.1
• Protein Refseq: NP_001239306.1
• UniProt ID: Q8TCD5