Active Recombinant Human NTRK1 protein, hFc-tagged

• Cat.No.: NTRK1-108H
• Product Overview: Active Recombinant Human NTRK1 protein(NP_002520.2) (Met1-Gly423) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-423 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Recombinant Human NGF / NGFB Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human TrkA Protein (ECD, Fc Tag), the EC50 is 4-12 ng/mL.
• Molecular Mass: The recombinant Human NTRK1 consisting of 629 amino acids and has a calculated molecular mass of 69.15 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 114.8 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NTRK1 neurotrophic tyrosine kinase, receptor, type 1 [ Homo sapiens ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; MTC; TRK; TRKA; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein; TRK1; Trk-A; p140-TrkA; DKFZp781I14186
• Gene ID: 4914
• mRNA Refseq: NM_001007792
• Protein Refseq: NP_001007793
• MIM: 191315
• UniProt ID: P04629