Recombinant Human PAFAH1B3 protein, His & T7-tagged

• Cat.No.: PAFAH1B3-6901H
• Product Overview: Recombinant Human PAFAH1B3 aa. (His28~Pro231 (Accession # Q15102)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with mental retardation, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.6kDa
• Protein length: His28~Pro231 (Accession # Q15102)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: PAFAH1B3 platelet activating factor acetylhydrolase 1b catalytic subunit 3 [ Homo sapiens (human) ]
• Official Symbol: PAFAH1B3
• Synonyms: PAFAH1B3; platelet activating factor acetylhydrolase 1b catalytic subunit 3; PAFAHG; platelet-activating factor acetylhydrolase IB subunit gamma; PAF acetylhydrolase 29 kDa subunit; PAF-AH 29 kDa subunit; PAF-AH subunit gamma; PAF-AH1b alpha 1 subunit; PAFAH subunit gamma; platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa); platelet-activating factor acetylhydrolase, isoform Ib, subunit 3 (29kDa)
• Gene ID: 5050
• mRNA Refseq: NM_001145939.1
• Protein Refseq: NP_001139411.1
• UniProt ID: Q15102