Recombinant Human PAFAH2 Protein, His-tagged

• Cat.No.: PAFAH2-039H
• Product Overview: Active recombinant N-terminal His-tagged human PAFAH2 protein (2-392) was expressed in E. coli.

Specification

• Description: This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 46.1 kDa
• Protein length: 2-392
• Purity: ≥80% as determined by SDS-PAGE
• Unit Definition: One unit is defined as the amount required to produce 1 nmol of TNB per minute at 37 centigrade in 50 mM HEPES, pH 7.4, containing 150 mM sodium chloride, 5 mM CHAPS, 1mM EDTA, 5% glycerol, and 300μM 2-thio PAF.
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM HEPES, pH 8.0, with 300 mM sodium chloride and 20% glycerol

Gene Information

• Gene Name: PAFAH2 platelet activating factor acetylhydrolase 2 [ Homo sapiens (human) ]
• Official Symbol: PAFAH2
• Synonyms: PAFAH2; platelet activating factor acetylhydrolase 2; platelet-activating factor acetylhydrolase 2, cytoplasmic; SD-PLA2; PAF:lysophospholipid transacetylase; AF:sphingosine transacetylase; platelet-activating factor acetylhydrolase 2, 40kDa; platelet-activating factor acetyltransferase PAFAH2; serine-dependent phospholipase A2; EC 2.3.1.149; EC 3.1.1.47
• Gene ID: 5051
• mRNA Refseq: NM_000437
• Protein Refseq: NP_000428
• MIM: 602344
• UniProt ID: Q99487