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Recombinant Human PAK1 Protein, GST-tagged

Cat.No. : PAK1-821H
Product Overview : Recombinant Human PAK1 protein Cdc42/Rac interactive binding region (AA 56-141) was expressed in E. coli with GST tag.
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Description : Members of the Rac/Rho subfamily of small GTPases or G-proteins are key regulators of numerous processes related to cytoskeletal dynamics. For example, Rac1/2 and cdc42 (and other closely related members) regulate cell migration, contraction and cell volume, among other features, through the control of the cortical actin cytoskeleton. Cdc42 is furthermore a major player in the establishment of a polarized phenotype in numerous specialized cell types whereas Rac is a critical regulator of NADPH oxidases and concomitant ROS production. In common with most other small G-proteins, cellular Rac and Cdc42 cycle between an inactive GDP-bound and an active GTP bound state. The CRIB domain of the Rac-effector PAK1 discriminates between these two conformations and can be used to isolate active Rac/Cdc42GTP complexes out of cell extracts.
Source : E. coli
Species : Human
Tag : GST
Form : PBS
Molecular Mass : 35 kDa
Purity : > 90 % (SDS PAGE), 30-50 % degradation (Please note: effective concentration of full-length polypeptide takes into account protein degradation that commonly accounts for 30-50 % of total protein)
Storage : Store at -80 centigrade, avoid freeze/thaw cycles., aliquot in convenient format upon first thawing.
Gene Name : PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
Official Symbol : PAK1
Synonyms : PAKalpha; PAK1
Gene ID : 5058
mRNA Refseq : NM_001128620.1
Protein Refseq : NP_001122092.1
MIM : 602590
UniProt ID : Q13153

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does PAK1 play in cancer progression and metastasis? 01/16/2022

PAK1 has been implicated in cancer progression due to its roles in cell survival, motility, and angiogenesis. Its overexpression has been observed in various tumors and is often associated with increased invasiveness and poor prognosis.

Are there any therapeutic implications related to targeting PAK1 in diseases? 07/17/2021

Given PAK1's involvement in cancer progression and other diseases, it has emerged as a potential therapeutic target. Several PAK1 inhibitors are under investigation for their potential in cancer therapy and other conditions where PAK1 signaling is dysregulated.

What are the downstream targets of PAK1 in signal transduction? 11/10/2018

Once activated, PAK1 phosphorylates a variety of substrates, including kinases, cytoskeletal proteins, and transcription factors. Some of its known targets include Raf-1, MEK1, and LIM kinases.

What is the primary function of the PAK1 protein in cellular signaling? 10/23/2018

PAK1 is a serine/threonine kinase that plays a crucial role in multiple cellular processes, including cytoskeletal reorganization, cell motility, and cell survival, primarily through its role in various signaling pathways.

How does PAK1 contribute to cytoskeletal dynamics? 06/17/2018

PAK1 regulates cytoskeletal dynamics by phosphorylating and modulating the activity of several cytoskeletal proteins, including filamin, LIM kinases, and myosin light chain, thereby influencing cell shape, motility, and adhesion.

How is PAK1 activated in cells? 01/24/2018

PAK1 is activated through binding to the GTP-bound forms of Cdc42 or Rac, small Rho GTPases. This binding induces a conformational change in PAK1, leading to its autophosphorylation and activation.

Are there any known genetic mutations in the PAK1 gene associated with diseases? 01/01/2018

While mutations in PAK1 are not commonly associated with hereditary diseases, its overexpression or hyperactivation has been linked to various cancers, promoting cell proliferation, survival, and migration.

Customer Reviews (3)

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Reviews
01/07/2020

    Worked as expected, perfect.

    01/23/2019

      Quick and efficient delivery.

      01/18/2018

        High purity, excellent yield.

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