Recombinant Human PAK1 Protein, GST-tagged
Cat.No. : | PAK1-821H |
Product Overview : | Recombinant Human PAK1 protein Cdc42/Rac interactive binding region (AA 56-141) was expressed in E. coli with GST tag. |
- Specification
- Gene Information
- Related Products
Description : | Members of the Rac/Rho subfamily of small GTPases or G-proteins are key regulators of numerous processes related to cytoskeletal dynamics. For example, Rac1/2 and cdc42 (and other closely related members) regulate cell migration, contraction and cell volume, among other features, through the control of the cortical actin cytoskeleton. Cdc42 is furthermore a major player in the establishment of a polarized phenotype in numerous specialized cell types whereas Rac is a critical regulator of NADPH oxidases and concomitant ROS production. In common with most other small G-proteins, cellular Rac and Cdc42 cycle between an inactive GDP-bound and an active GTP bound state. The CRIB domain of the Rac-effector PAK1 discriminates between these two conformations and can be used to isolate active Rac/Cdc42GTP complexes out of cell extracts. |
Source : | E. coli |
Species : | Human |
Tag : | GST |
Form : | PBS |
Molecular Mass : | 35 kDa |
Purity : | > 90 % (SDS PAGE), 30-50 % degradation (Please note: effective concentration of full-length polypeptide takes into account protein degradation that commonly accounts for 30-50 % of total protein) |
Storage : | Store at -80 centigrade, avoid freeze/thaw cycles., aliquot in convenient format upon first thawing. |
Gene Name : | PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ] |
Official Symbol : | PAK1 |
Synonyms : | PAKalpha; PAK1 |
Gene ID : | 5058 |
mRNA Refseq : | NM_001128620.1 |
Protein Refseq : | NP_001122092.1 |
MIM : | 602590 |
UniProt ID : | Q13153 |
Products Types
◆ Recombinant Protein | ||
Pak1-4662M | Recombinant Mouse Pak1 Protein, Myc/DDK-tagged | +Inquiry |
PAK1-5512H | Recombinant Human Protein (Cdc42/Rac)-Activated Kinase 1, GST-tagged | +Inquiry |
PAK1-3921R | Recombinant Rat PAK1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PAK1-768H | Recombinant Human PAK1 Protein, His-tagged | +Inquiry |
PAK1-4259R | Recombinant Rat PAK1 Protein | +Inquiry |
◆ Lysates | ||
PAK1-1275HCL | Recombinant Human PAK1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionPAK1 has been implicated in cancer progression due to its roles in cell survival, motility, and angiogenesis. Its overexpression has been observed in various tumors and is often associated with increased invasiveness and poor prognosis.
Given PAK1's involvement in cancer progression and other diseases, it has emerged as a potential therapeutic target. Several PAK1 inhibitors are under investigation for their potential in cancer therapy and other conditions where PAK1 signaling is dysregulated.
Once activated, PAK1 phosphorylates a variety of substrates, including kinases, cytoskeletal proteins, and transcription factors. Some of its known targets include Raf-1, MEK1, and LIM kinases.
PAK1 is a serine/threonine kinase that plays a crucial role in multiple cellular processes, including cytoskeletal reorganization, cell motility, and cell survival, primarily through its role in various signaling pathways.
PAK1 regulates cytoskeletal dynamics by phosphorylating and modulating the activity of several cytoskeletal proteins, including filamin, LIM kinases, and myosin light chain, thereby influencing cell shape, motility, and adhesion.
PAK1 is activated through binding to the GTP-bound forms of Cdc42 or Rac, small Rho GTPases. This binding induces a conformational change in PAK1, leading to its autophosphorylation and activation.
While mutations in PAK1 are not commonly associated with hereditary diseases, its overexpression or hyperactivation has been linked to various cancers, promoting cell proliferation, survival, and migration.
Customer Reviews (3)
Write a reviewWorked as expected, perfect.
Quick and efficient delivery.
High purity, excellent yield.
Ask a Question for All PAK1 Products
Required fields are marked with *
My Review for All PAK1 Products
Required fields are marked with *
Inquiry Basket