Recombinant Human PCBP1 Protein, His-tagged

• Cat.No.: PCBP1-327H
• Product Overview: Recombinant human PCBP1 (NP_006187.2) (Asp 2-Cys 356) was expressed, with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant human PCBP1 comprises 362 amino acids and has a predicted molecular mass of 38.3 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >87 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PCBP1 poly(rC) binding protein 1 [ Homo sapiens ]
• Official Symbol: PCBP1
• Synonyms: PCBP1; poly(rC) binding protein 1; poly(rC)-binding protein 1; heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; hnRNP X; HNRPE1; HNRPX; alpha-CP1; nucleic acid-binding protein SUB2.3; heterogenous nuclear ribonucleoprotein X; heterogenous nuclear ribonucleoprotein E1; hnRNP-X; hnRNP-E1
• Gene ID: 5093
• mRNA Refseq: NM_006196
• Protein Refseq: NP_006187
• MIM: 601209
• UniProt ID: Q15365
• Chromosome Location: 2p13-p12
• Pathway: Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem