Recombinant Human PCSK1 protein, His-tagged

• Cat.No.: PCSK1-892H
• Product Overview: Recombinant Human PCSK1 protein(NP_000430.3)(Met1-Arg617), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg617
• Form: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The purified recombinant human PCSK1 consists of 518 amino acids after removal of the pro peptide due to autocatalytic processing, corresponding to the active form (aa 111-617), and has a predicted molecular mass of 57.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the active rh PCSK1 is approximately 66 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PCSK1 proprotein convertase subtilisin/kexin type 1 [ Homo sapiens ]
• Official Symbol: PCSK1
• Synonyms: PCSK1; proprotein convertase subtilisin/kexin type 1; NEC1; neuroendocrine convertase 1; PC1; PC3; prohormone convertase 1; prohormone convertase 3; proprotein convertase 1; SPC3; NEC 1; BMIQ12
• Gene ID: 5122
• mRNA Refseq: NM_000439
• Protein Refseq: NP_000430
• MIM: 162150
• UniProt ID: P29120