Recombinant Human PCSK1 protein, His-tagged

• Cat.No.: PCSK1-892H
• Product Overview: Recombinant Human PCSK1 protein(NP_000430.3) (Met 1-Arg 617) was expressed in Human Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 1-617 aa
• Form: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5.
• Molecular Mass: The purified recombinant human PCSK1 consists of 518 amino acids after removal of the pro peptide due to autocatalytic processing, corresponding to the active form (aa 111-617), and has a predicted molecular mass of 57.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the active rh PCSK1 is approximately 66 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PCSK1 proprotein convertase subtilisin/kexin type 1 [ Homo sapiens ]
• Official Symbol: PCSK1
• Synonyms: PCSK1; proprotein convertase subtilisin/kexin type 1; NEC1; neuroendocrine convertase 1; PC1; PC3; prohormone convertase 1; prohormone convertase 3; proprotein convertase 1; SPC3; NEC 1; BMIQ12
• Gene ID: 5122
• mRNA Refseq: NM_000439
• Protein Refseq: NP_000430
• MIM: 162150
• UniProt ID: P29120