Active Recombinant Human PCSK9 protein(with mutation Asp 374 Tyr), His-tagged

• Cat.No.: PCSK9-312H
• Product Overview: Active Recombinant Human PCSK9 protein(NP_777596.2, with mutation Asp 374 Tyr) (Met 1-Gln 692) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-692 aa, with mutation Asp 374 Tyr
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human PCSK9-His(D374Y)at 10 μg/ml (100 μl /well) can bind biotinylated LDLR-His, The EC50 of biotinylated LDLR-His is 20-60ng/ml.
• Molecular Mass: The recombinant human PCSK9 consists 673 amino acids and predicts a molecular mass of 72.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > (76.6 + 19.4)% as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells (2015)

Gene Information

• Gene Name: PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens ]
• Official Symbol: PCSK9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; HCHOLA3, hypercholesterolemia, autosomal dominant 3; FH3; NARC 1; NARC1; NARC-1; HCHOLA3
• Gene ID: 353175
• MIM: 607786
• UniProt ID: Q8NBP7