Recombinant Human PDCD1 Protein, Fc-Avi-His-tagged, Biotinylated PDCD1-258H

Recombinant Human PDCD1 Protein, Fc-Avi-His-tagged, Biotinylated

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Cat.No. : PDCD1-258H Optional Service: Optional requirements on this protein
Product Overview : Biotinylated Human PD-1 / PDCD1, His Tag & Fc Tag is expressed from HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_005009). This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag.
Source : HEK293
Species : Human
Tag : Fc-Avi-His
Predicted N Terminal : Leu 25
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 ul/well),can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 ug/mL. Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, His Tag, Fc tag can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1ug/mL. FACS analysis shows that the binding of Biotinylated Human PD-1, His Tag, Fc tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD1 used is 1ug/mL. The IC50 is 0.362 ug/mL.
Molecular Mass : The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation and 100-130 kDa under non-reducing condition.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : PDCD1 programmed cell death 1 [ Homo sapiens ]
Official Symbol : PDCD1
Synonyms : PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l;
Gene ID : 5133
mRNA Refseq : NM_005018
Protein Refseq : NP_005009
MIM : 600244
UniProt ID : Q15116
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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