| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc-Avi-His |
| Predicted N Terminal : |
Leu 25 |
| Form : |
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : |
Immobilized Human PD-L1, Fc Tag at 5 ug/mL (100 ul/well),can bind Biotinylated Human PD-1, His Tag & Fc Tag with a linear range of 0.01-0.1 ug/mL. Flow Cytometry assay shows that recombinant Biotinylated Human PD-1, His Tag, Fc tag can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1ug/mL. FACS analysis shows that the binding of Biotinylated Human PD-1, His Tag, Fc tag to 293 overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-PD-L1 antibody. The concentration of PD1 used is 1ug/mL. The IC50 is 0.362 ug/mL. |
| Molecular Mass : |
The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation and 100-130 kDa under non-reducing condition. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Characteristic : |
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
