| Description : |
Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized |
| Molecular Mass : |
Recombinant protein has a calculated molecular weight of about 16 kDa. Due to the abundant glycosylation, it migrates as approximately 30-40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Endotoxin : |
Not determined |
| Purity : |
>95% |
| Applications : |
Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc. |
| Usage : |
This product is furnished for LABORATORY RESEARCH USE ONLY.Not for diagnostic or therapeutic use. |
| Storage : |
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles. |
| Concentration : |
Determined by Pierce BCA protein assay kit |
| Storage Buffer : |
Lyophilized from a 0.2 µm filtered solution in PBS |
| Preservative : |
None |
| Reconstitution : |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Shipping : |
Ice packs |
| Purity determined by : |
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Purification : |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
| Expressed Region : |
Pro21-Val170 |
