| Cat.No. : |
PDK1-124H |
| Product Overview : |
Recombinant full-length human PDHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Description : |
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. |
| Source : |
Insect cells |
| Species : |
Human |
| Tag : |
GST |
| Bio-activity : |
1.7 nmol/min/mg |
| Molecular Mass : |
~70 kDa |
| Purity : |
>95% |
| Stability : |
One year at -70 centigrade from the date of shipment. |
| Storage : |
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Concentration : |
0.1 μg/μL |
| Storage Buffer : |
Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Specific Activity : |
The specific activity of PDK1 was determined to be 1.7 nmol/min/mg as per activity assay protocol.
|
| Purity : |
The purity of PDK1 was determined to be >95% by densitometry.
|
