Recombinant Human PDK1 protein(Ser29-Ala436), His-tagged

• Cat.No.: PDK1-904H
• Product Overview: Recombinant Human PDK1 (NP_002601.1) (Ser 29-Ala 436) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: Ser29-Ala436
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PDK1 consists of 427 amino acids and has a calculated molecular mass of 48.6 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDK1 pyruvate dehydrogenase kinase, isozyme 1 [ Homo sapiens ]
• Official Symbol: PDK1
• Synonyms: PDK1; pyruvate dehydrogenase kinase, isozyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• Gene ID: 5163
• mRNA Refseq: NM_002610
• Protein Refseq: NP_002601
• MIM: 602524
• UniProt ID: Q15118

Reviews

07/22/2022

PDK1 production process is strictly controlled and the quality of the product is guaranteed.

08/08/2020

Reproducibility of PDK1 is simply unrivaled, with identical results in every experiment or application, demonstrating its high stability and reliability.

09/15/2019

The catalytic activity of PDK1 is excellent, which can simplify complex chemical reactions and improve yields, and its scientific research value is self-evident.

Q&As

Is PDK1 associated with obesity?

May play a role in the occurrence of obesity, and the current research conclusions still need to be further verified.

Is PDK1 related to the cardiovascular system?

PDK1 is thought to be associated with the occurrence and progression of cardiovascular disease, and may affect the health of the cardiovascular system by regulating the glucose metabolism of cardiomyocytes and the activity of phosphatase. However, further research and validation are needed.

What signal pathways does PDK1 participate in and how does it regulate signals?

Participates in a variety of signaling pathways, such as Akt and PKC, and affects the transmission and regulation of various signaling pathways by regulating the kinase activities of related receptors and enzymes.

In which tumors are PDK1 abnormally expressed?

PDK1 has abnormal expression in a variety of tumors, such as breast cancer, pancreatic cancer, colorectal cancer, etc., and is one of the important markers of tumor occurrence and prognosis.

How does PDK1 regulate cell metabolism?

The plays an important role in regulating cell metabolism and plays an important role in energy metabolism by regulating the phosphorylation state of various enzymes.

Can PDK1 be used as a target for the treatment of new diabetes?

It has been identified as a potential therapeutic target for new diabetes, possibly by improving insulin signaling and glycogen synthesis, but further exploration is needed.