Recombinant Human PDP1, MYC/DDK-tagged

• Cat.No.: PDP1-695H
• Product Overview: Recombinant Human phosphatase 2C, magnesium-dependent, catalytic subunit (PPM2C), nuclear gene encoding mitochondrial protein, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.

Specification

• Description: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: HEK293 cells
• Species: Human
• Tag: MYC/DDK
• Molecular Mass: 60.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: PDP1 pyruvate dehyrogenase phosphatase catalytic subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PDP1
• Synonyms: PDP1; pyruvate dehyrogenase phosphatase catalytic subunit 1; PDP 1; PDPC 1; [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; protein phosphatase 2C, magnesium-dependent, catalytic subunit; pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase; pyruvate dehydrogenase phosphatase catalytic subunit 1; PDH; PDP; PDPC; PPM2C; FLJ32517; FLJ56179; MGC119646; EC 3.1.3.43
• Gene ID: 54704
• mRNA Refseq: NM_018444
• Protein Refseq: NP_060914
• MIM: 605993
• UniProt ID: Q9P0J1
• Chromosome Location: 8q22.
• Pathway: Metabolism; Pyruvate metabolism; Regulation of pyruvate dehydrogenase (PDH) complex
• Function: [pyruvate dehydrogenase (lipoamide)] phosphatase activity; metal ion binding; protein serine/threonine phosphatase activity