| Description : |
Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His & T7 |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
Predicted Molecular Mass: 29.6kDa |
| Protein length : |
Asn315~Glu537 (Accession # Q9P0J1) |
| Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
| Purity : |
>95% |
| Characteristic : |
The isoelectric point is 6.8. |
| Applications : |
SDS-PAGE; WB; ELISA; IP |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl |
| Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |
