Recombinant Human PFDN5 protein, His-tagged

• Cat.No.: PFDN5-3608H
• Product Overview: Recombinant Human PFDN5(1-154aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-154 a.a.
• Description: PFDN5 belongs to the prefoldin alpha subunit family. Prefoldin (PFDN), a ubiquitously expressed heterohexameric co-chaperone, is necessary for proper folding of nascent proteins, in particular, tubulin and actin. PFDN5 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. This protein may also repress the transcriptional activity of the proto-oncogene c-Myc.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol
• Molecular Mass: 19.5kDa (174aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHYVPGKLHDVE HVLIDVGTGY YVEKTAEDAK DFFKRKIDFL TKQMEKIQPA LQEKHAMKQA VMEMMSQKIQ QLTALGAAQA TAKAMAQSINITEL NLPQLEMLKN QLDQEVEFLS TSIAQLKVVQ TKYVEAKDCL NVLNKSNEGK ELLVPLTSSM
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Publications:
  Prefoldin 5 and anti-prefoldin 5 antibodies as biomarkers for uveitis in ankylosing spondylitis (2019)

Gene Information

• Gene Name: PFDN5 prefoldin subunit 5 [ Homo sapiens ]
• Official Symbol: PFDN5
• Synonyms: PFDN5; prefoldin subunit 5; prefoldin 5; MM 1; PFD5; myc modulator-1; c-myc binding protein; MM1; MM-1; MGC5329; MGC71907
• Gene ID: 5204
• mRNA Refseq: NM_002624
• Protein Refseq: NP_002615
• MIM: 604899
• UniProt ID: Q99471
• Chromosome Location: 12q13.13
• Pathway: Chaperonin-mediated protein folding, organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Prefoldin mediated transfer of substrateto CCT/TriC, organism-specific biosystem; Protein folding, organism-specific biosystem
• Function: protein binding; transcription corepressor activity; unfolded protein binding

Citation

Prefoldin 5 and Anti-prefoldin 5 Antibodies as Biomarkers for Uveitis in Ankylosing Spondylitis

Journal: Frontiers in Immunology    PubMed ID: 30891043    Data: 2019/3/5

Authors: Oh Chan Kwon, Eun-Ju Lee, Yong-Gil Kim

Article Snippet:Anti-PFDN5 was also measured by ELISA.Anti-PFDN5 was also measured by ELISA.. Ninety-six well plates were coated with 1 μg/mL of recombinant human PFDN5 (Creative BioMart).. Diluted serum (1:200) were added and were incubated for 2 h, followed by addition of secondary antibodies (goat anti-human IgG, Jackson ImmunoResearch).Diluted serum (1:200) were added and were incubated for 2 h, followed by addition of secondary antibodies (goat anti-human IgG, Jackson ImmunoResearch).

Association between anti-PFDN5 antibody and AS with uveitis. (A) Protein microarray analysis of autoreactivity to PFDN5 in sera from patients with various autoimmune diseases in the Multiple Autoimmune Disease Genetics Consortium Cohort. (B) Receiver operating characteristic curve for anti-PFDN5 as a biomarker of uveitis in AS. (C) ELISA analysis of anti-PFDN5 antibody levels in sera from Korean cohort. (D) ELISA analysis of PFDN5 protein levels in sera from Korean cohort. Data presented as mean ± SEM. * p < 0.05; ** p < 0.01, by Mann–Whitney U -test. PFDN5, prefoldin subunit 5; RA, rheumatoid arthritis; CCP, cyclic citrullinated protein; JIA, juvenile idiopathic arthritis; PsA, psoriatic arthritis; PAH, pulmonary artery hypertension; AUC, area under the curve; HC, healthy control; ELISA, enzyme-linked immunosorbent assay; ns, not significant.

Expression of PFDN5 in curdlan-treated SKG mice with ocular lesions, and the role of PFDN5 against apoptosis in ARPE19 cells. (A) The experimental design. (B) Comparison of gross phenotype, microscopic ophthalmologic exam, H&E staining, IHC, and TUNEL assay between PBS-treated SKG mice and curdlan-treated-SKG mice. (C) ELISA analysis of anti-PFDN5 antibody levels in sera of PBS-treated SKG mice and curdlan-treated SKG mice according to the number of weeks post-injection. (D) Western blot showing protein expression of PFDN5 in ARPE19 cells transfected with non-targeting scrambled siRNA, scrambled siRNA with tunicamycin stimulation, siRNA against PFDN5 , and si- PFDN5 with tunicamycin stimulation. (E) Representative flow cytometry plot of 7-AAD and annexin V staining for ARPE19 cells transfected with non-targeting scrambled siRNA, scrambled siRNA with tunicamycin stimulation, siRNA against PFDN5 , and si- PFDN5 with tunicamycin stimulation. (F) Comparison of the proportion of necrotic cells or early apoptotic cells among ARPE19 cells transfected with scrambled siRNA, scrambled siRNA under tunicamycin stimulation, si-PFDN5 and si-PFDN5 under tunicamycin stimulation. Data presented as mean ± SEM. * p < 0.05; *** p < 0.001, by Mann–Whitney U -test. H&E, hematoxylin and eosin; PFDN5, prefoldin subunit 5; IHC, immunohistochemistry; TUNEL, Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling; ELISA, enzyme-linked immunosorbent assay; ARPE19, retinal pigment epithelial 19; 7-AAD, 7-aminoactinomycin D; ns, not significant.