Recombinant Human PFKM Protein, His-tagged

• Cat.No.: PFKM-2582H
• Product Overview: Recombinant Human PFKM Protein (Thr2-His390) with a N-His tag was expressed in E. coli.

Specification

• Description: Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 46.3 kDa
  Accurate Molecular Mass: 46 kDa
• Protein length: Thr2-His390
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PFKM phosphofructokinase, muscle [ Homo sapiens (human) ]
• Official Symbol: PFKM
• Synonyms: PFKM; phosphofructokinase, muscle; PFKX, phosphofructokinase, polypeptide X; 6-phosphofructokinase, muscle type; PFK 1; PFK-A; phosphohexokinase; phosphofructokinase 1; phosphofructokinase-M; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase, polypeptide X; GSD7; PFK1; PFKA; PFKX; PFK-1; MGC8699
• Gene ID: 5213
• mRNA Refseq: NM_000289
• Protein Refseq: NP_000280
• MIM: 610681
• UniProt ID: P08237