Active Recombinant Human PGK1 protein, His-tagged

• Cat.No.: PGK1-191H
• Product Overview: Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris (pH 8.0), containing 10% Glycerol, 1mM DTT.
• Bio-activity: Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
• Molecular Mass: 46.8 kDa (437aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PGK1 phosphoglycerate kinase 1 [ Homo sapiens ]
• Official Symbol: PGK1
• Synonyms: PGK1; phosphoglycerate kinase 1; PRP 2; primer recognition protein 2; cell migration-inducing gene 10 protein; PGKA; MIG10; MGC8947; MGC117307; MGC142128
• Gene ID: 5230
• mRNA Refseq: NM_000291
• Protein Refseq: NP_000282
• MIM: 311800
• UniProt ID: P00558
• Chromosome Location: Xq13.3
• Pathway: Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem
• Function: ATP binding; nucleotide binding; phosphoglycerate kinase activity; phosphoglycerate kinase activity; transferase activity