Recombinant Human Phosphomannomutase 1, His-tagged

• Cat.No.: PMM1-1422H
• Product Overview: PMM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 282 amino acids (1-262 a.a.) and having a molecular mass of 31.9 kDa. The PMM1 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Protein Length: 1-262 a.a.
• Description: PMM1 is an enzyme involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM1 catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for the synthesis of dolichol-phosphate-mannose, which is crucial for N-linked glycosylation and accordingly the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins.
• Form: The PMM1 solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 100 mM NaCl and 0.1 mM PMSF.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered colorless solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA
• Storage: PMM1 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PMM1 phosphomannomutase 1 [ Homo sapiens ]
• Official Symbol: PMM1
• Synonyms: PMM1; phosphomannomutase 1; Sec53; phosphomannomutase 1; PMM 1; PMMH-22; OTTHUMP00000198843; brain glucose-1,6-bisphosphatase
• Gene ID: 5372
• mRNA Refseq: NM_002676
• Protein Refseq: NP_002667
• MIM: 601786
• UniProt ID: Q92871
• Chromosome Location: 22q13
• Pathway: Amino sugar and nucleotide sugar metabolism; Asparagine N-linked glycosylation; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein; Fructose and mannose metabolism; Metabolic pathways; Metabolism of proteins; Post-translational protein modification; Synthesis of GDP-mannose; Synthesis of substrates in N-glycan biosythesis
• Function: isomerase activity; metal ion binding; phosphomannomutase activity