Recombinant Human PIGR Protein, His-tagged

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Recombinant Human PIGR Protein, His-tagged

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Cat.No. : PIGR-013H
Product Overview : Recombinant human pIgR, fused to His-tag at C terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description : This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.
Source : Insect cells
Species : Human
Tag : His
Form : Liquid
Bio-activity : Measured by its binding ability in a functional ELISA with Human IgM. The ED50 range ≤10 μg/mL.
Molecular Mass : 68.9 kDa
AA Sequence : <ADL>KSPIF GPEEVNSVEG NSVSITCYYP PTSVNRHTRK YWCRQGARGG CITLISSEGY VSSKYAGRAN LTNFPENGTF VVNIAQLSQD DSGRYKCGLG INSRGLSFDV SLEVSQGPGL LNDTKVYTVD LGRTVTINCP FKTENAQKRK SLYKQIGLYP VLVIDSSGYV NPNYTGRIRL DIQGTGQLLF SVVINQLRLS DAGQYLCQAG DDSNSNKKNA DLQVLKPEPE LVYEDLRGSV TFHCALGPEV ANVAKFLCRQ SSGENCDVVV NTLGKRAPAF EGRILLNPQD KDGSFSVVIT GLRKEDAGRY LCGAHSDGQL QEGSPIQAWQ LFVNEESTIP RSPTVVKGVA GGSVAVLCPY NRKESKSIKY WCLWEGAQNG RCPLLVDSEG WVKAQYEGRL SLLEEPGNGT FTVILNQLTS RDAGFYWCLT NGDTLWRTTV EIKIIEGEPN LKVPGNVTAV LGETLKVPCH FPCKFSSYEK YWCKWNNTGC QALPSQDEGP SKAFVNCDEN SRLVSLTLNL VTRADEGWYW CGVKQGHFYG ETAAVYVAVE ERKAAGSRDV SLAKADAAPD EKVLDSGFRE IENKAIQDPR LFAEEKAVAD TRDQADGSRA SVDSGSSEEQ GGSSR<HHHH HH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 95% by SDS-PAGE
Applications : SDS-PAGE, Bioactivity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -92 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/mL (determined by Absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity :

Human IgM is coated at 2 μg/mL (100 μL/well) can bind Human pIgR. The ED50 range ≤10 μg/mL.

Gene Name : PIGR polymeric immunoglobulin receptor [ Homo sapiens (human) ]
Official Symbol : PIGR
Synonyms : PIGR; polymeric immunoglobulin receptor; polymeric immunoglobulin receptor; hepatocellular carcinoma associated protein TB6; poly-Ig receptor
Gene ID : 5284
mRNA Refseq : NM_002644
Protein Refseq : NP_002635
MIM : 173880
UniProt ID : P01833

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