Recombinant Human PIK3AP1 293 Cell Lysate
Cat.No. : | PIK3AP1-3192HCL |
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Species : | Human |
Source : | HEK293 |
Tag : | Non |
Description : | Antigen standard for phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name | PIK3AP1 phosphoinositide-3-kinase adaptor protein 1 [ Homo sapiens ] |
Official Symbol | PIK3AP1 |
Synonyms | PIK3AP1; phosphoinositide-3-kinase adaptor protein 1; phosphoinositide 3-kinase adapter protein 1; BCAP; FLJ35564; B cell adaptor protein; B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; RP11-34E5.3; |
Gene ID | 118788 |
mRNA Refseq | NM_152309 |
Protein Refseq | NP_689522 |
MIM | 607942 |
UniProt ID | Q6ZUJ8 |
Chromosome Location | 10q24.2 |
Pathway | Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; Immune System, organism-specific biosystem; Signaling by the B Cell Receptor (BCR), organism-specific biosystem; |
Function | identical protein binding; |
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Not For Human Consumption!
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