Active Recombinant Human PIK3CA and Mouse Pik3ca protein, His-tagged
Cat.No. : | PIK3R1&Pik3ca-155H |
Product Overview : | Recombinant full-length mouse p110 alpha and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins. |
- Specification
- Gene Information
- Related Products
Description : | The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling. |
Source : | Insect cells |
Species : | Human |
Tag : | His |
Form : | 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
Bio-activity : | 3600 nmol/min/mg |
Molecular Mass : | p110 alpha ~111 kDa and p85 alpha ~86 kDa |
Purity : | >70% |
Applications : | Kinase Assay |
Stability : | 1 year at -70 centigrade from the date of shipment |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1μg/μl |
Gene Name : | PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ] |
Official Symbol : | PIK3R1 |
Synonyms : | PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; p85; p85 ALPHA; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); p85-ALPHA; |
Gene ID : | 5295 |
mRNA Refseq : | NM_181523 |
Protein Refseq : | NP_852664 |
MIM : | 171833 |
UniProt ID : | P27986 |
Chromosome Location : | 5q13.1 |
Pathway : | 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; |
Function : | 1-phosphatidylinositol binding; ErbB-3 class receptor binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor receptor binding; neurotrophin TRKA receptor binding; phosphatidylinositol 3-kinase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein phosphatase binding; |
Products Types
◆ Recombinant Protein | ||
PIK3R1-4123R | Recombinant Rat PIK3R1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PIK3R1-4934H | Recombinant Human PIK3R1 Protein, His-tagged | +Inquiry |
Pik3r1-4865M | Recombinant Mouse Pik3r1 Protein, Myc/DDK-tagged | +Inquiry |
PIK3R1-3252R | Recombinant Rhesus Macaque PIK3R1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PIK3R1-29688TH | Recombinant Human Human PIK3CA, His-tagged | +Inquiry |
◆ Lysates | ||
PIK3R1-3185HCL | Recombinant Human PIK3R1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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