Recombinant Human PIK3CD protein, His & T7-tagged

• Cat.No.: PIK3CD-1727H
• Product Overview: Recombinant Human PIK3CD aa. (Val774~Gln1044 (Accession # O00329)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.8kDa
• Protein length: Val774~Gln1044 (Accession # O00329)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 8.7

Gene Information

• Gene Name: PIK3CD phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta [ Homo sapiens (human) ]
• Official Symbol: PIK3CD
• Synonyms: PIK3CD; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; APDS; PI3K; IMD14; p110D; P110DELTA; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase p110 subunit delta; PI3Kdelta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide-3-kinase C; phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta; ptdIns-3-kinase subunit p110-delta
• Gene ID: 5293
• mRNA Refseq: NM_005026.4
• Protein Refseq: NP_005017.3
• UniProt ID: O00329