Recombinant Human PKM2 Protein, His&GST-tagged

• Cat.No.: PKM2-1942H
• Product Overview: Recombinant Human PKM2 Protein (Met1-Pro531) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 87.9 kDa
  Accurate Molecular Mass: 88 kDa
• Protein length: Met1-Pro531
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PKM2 pyruvate kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: PKM2
• Synonyms: PKM2; pyruvate kinase, muscle; pyruvate kinase isozymes M1/M2; OIP3; PK3; THBP1; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic; PKM; TCB; CTHBP; MGC3932
• Gene ID: 5315
• mRNA Refseq: NM_001206797
• Protein Refseq: NP_001193726
• MIM: 179050
• UniProt ID: P14618