Recombinant Human PKNOX1, His-tagged

• Cat.No.: PKNOX1-208H
• Product Overview: PKNOX1, 1-436, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-436aa
• Description: Hmeobox protein PKNOX1 is a DNA-binding protein that forms stable complexes with PBX proteins which synergize with AP-1 binding factors to augment transcription of the urokinase gene. Also referred to as UEF3, PRP-1 or p64, PKNOX1 appears to be a general DNA-binding factor involved in modulating the transcriptional activity of AP-1 containing promoters. Recombinant human PKNOX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Molecular Mass: 49.7 kDa (456aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSEPDAEGV SPPPVESQTP MDVDKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKEGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTISPQGIV VPASALQQGN VAMATVAGGT VYQPVTVVTP QGQVVTQTLS PGTIRIQNSQ LQLQLNQDLS ILHQDDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKTAQNR PVQRFWPDSI ASGVAQPPPS ELTMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM AGQSEDESVD STEEDAGALA PAHISGLVLE NSDSLQ
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PKNOX1 PBX/knotted 1 homeobox 1 [ Homo sapiens ]
• Official Symbol: PKNOX1
• Synonyms: PKNOX1; PBX/knotted 1 homeobox 1; homeobox protein PKNOX1; PREP1; PBX/knotted homeobox 1; homeobox protein PREP-1; Pbx regulating protein-1; human homeobox-containing protein; pkonx1c
• Gene ID: 5316
• mRNA Refseq: NM_004571
• Protein Refseq: NP_004562
• MIM: 602100
• UniProt ID: P55347
• Chromosome Location: 21q22.3
• Function: protein heterodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity