Recombinant Human PLA2G15 Protein, His-tagged

• Cat.No.: PLA2G15-2592H
• Product Overview: Recombinant Human PLA2G15 Protein (Ser132-Pro412) with a N-His tag was expressed in E. coli.

Specification

• Description: Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 35.7 kDa
  Accurate Molecular Mass: 36 kDa
• Protein length: Ser132-Pro412
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: PLA2G15 phospholipase A2, group XV [ Homo sapiens (human) ]
• Official Symbol: PLA2G15
• Synonyms: PLA2G15; phospholipase A2, group XV; LYPLA3, lysophospholipase 3 (lysosomal phospholipase A2); group XV phospholipase A2; GXVPLA2; LLPL; 1-O-acylceramide synthase; lysosomal phospholipase A2; LCAT-like lysophospholipase; lysophospholipase 3 (lysosomal phospholipase A2); ACS; LPLA2; LYPLA3; DKFZp564A0122
• Gene ID: 23659
• mRNA Refseq: NM_012320
• Protein Refseq: NP_036452
• MIM: 609362
• UniProt ID: Q8NCC3