Recombinant Human PLAT Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PLAT Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PLAT-1553H
Product Overview : PLAT MS Standard C13 and N15-labeled recombinant protein (NP_127509) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 57.4 kDa
AA Sequence : MDAMKRGLCC VLLLCGAVFV SPSQEIHARF RRGARSYQGC SEPRCFNGGT CQQALYFSDF VCQCPEGFAG KCCEIDTRAT CYEDQGISYR GTWSTAESGA ECTNWNSSAL AQKPYSGRRP DAIRLGLGNH NYCRNPDRDS KPWCYVFKAG KYSSEFCSTP ACSEGNSDCY FGNGSAYRGT HSLTESGASC LPWNSMILIG KVYTAQNPSA QALGLGKHNY CRNPDGDAKP WCHVLKNRRL TWEYCDVPSC STCGLRQYSQ PQFRIKGGLF ADIASHPWQA AIFAKHRRSP GERFLCGGIL ISSCWILSAA HCFQERFPPH HLTVILGRTY RVVPGEEEQK FEVEKYIVHK EFDDDTYDND IALLQLKSDS SRCAQESSVV RTVCLPPADL QLPDWTECEL SGYGKHEALS PFYSERLKEA HVRLYPSSRC TSQHLLNRTV TDNMLCAGDT RSGGPQANLH DACQGDSGGP LVCLNDGRMT LVGIISWGLG CGQKDVPGVY TKVTNYLDWI RDNMRPTRTR PLEQKLISEE DLAANDILDY KDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PLAT plasminogen activator, tissue type [ Homo sapiens (human) ]
Official Symbol : PLAT
Synonyms : PLAT; plasminogen activator, tissue; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); TPA; T-PA; DKFZp686I03148;
Gene ID : 5327
mRNA Refseq : NM_033011
Protein Refseq : NP_127509
MIM : 173370
UniProt ID : P00750

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