| Description : |
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
83.6 kDa |
| AA Sequence : |
MRPLLLLALLGWLLLAEAKGDAKPE DNLLVLTVATKETEGFRRFKRSAQF FNYKIQALGLGEDWNVEKGTSAGGG QKVRLLKKALEKHADKEDLVILFTD SYDVLFASGPRELLKKFRQSRSQVV FSAEELIYPDRRLETKYPVVSDGKR FLGSGGFIGYAPNLSKLVAEWEGQD SDSDQLFYTKIFLDPEKREQINITL DHRCRIFQNLDGALDEVVLKFEMGH VRARNLAYDTLPVLIHGNGPTKLQL NYLGNYIPRFWTFETGCTVCDEGLR SLKGIGDEALPTVLVGVFIEQPTPF VSLFFQRLLRLHYPQKHMRLFIHNH EQHHKAQVEEFLAQHGSEYQSVKLV GPEVRMANADARNMGADLCRQDRSC TYYFSVDADVALTEPNSLRLLIQQN KNVIAPLMTRHGRLWSNFWGALSAD GYYARSEDYVDIVQGRRVGVWNVPY ISNIYLIKGSALRGELQSSDLFHHS KLDPDMAFCANIRQQDVFMFLTNRH TLGHLLSLDSYRTTHLHNDLWEVFS NPEDWKEKYIHQNYTKALAGKLVET PCPDVYWFPIFTEVACDELVEEMEH FGQWSLGNNKDNRIQGGYENVPTID IHMNQIGFEREWHKFLLEYIAPMTE KLYPGYYTRAQFDLAFVVRYKPDEQ PSLMPHHDASTFTINIALNRVGVDY EGGGCRFLRYNCSIRAPRKGWTLMH PGRLTHYHEGLPTTRGTRYIAVSFV DPTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
