Recombinant Human PMEL Protein, Tag Free

• Cat.No.: PMEL-01H
• Product Overview: Recombinant human PMEL protein without tag was expressed in E. coli

Specification

• Description: This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: Tag Free
• Molecular Mass: The protein has a calculated MW of 60.5 kDa.
• AA Sequence: MGATKVPRNQDWLGVSRQLRTKAWN RQLYPEWTEAQRLDCWRGGQVSLKV SNDGPTLIGANASFSIALNFPGSQK VLPDGQVIWVNNTIINGSQVWGGQP VYPQETDDACIFPDGGPCPSGSWSQ KRSFVYVWKTWGQYWQVLGGPVSGL SIGTGRAMLGTHTMEVTVYHRRGSR SYVPLAHSSSAFTITDQVPFSVSVS QLRALDGGNKHFLRNQPLTFALQLH DPSGYLAEADLSYTWDFGDSSGTLI SRALVVTHTYLEPGPVTAQVVLQAA IPLTSCGSSPVPGTTDGHRPTAEAP NTTAGQVPTTEVVGTTPGQAPTAEP SGTTSVQVPTTEVISTAPVQMPTAE STGMTPEKVPVSEVMGTTLAEMSTP EATGMTPAEVSIVVLSGTTAAQVTT TEWVETTARELPIPEPEGPDASSIM STESITGSLGPLLDGTATLRLVKRQ VPLDCVLYRYGSFSVTLDIVQGIES AEILQAVPSGEGDAFELTVSCQGGL PKEACMEISSPGCQPPAQRLCQPVL PSPACQLVLHQILKGGSGTYCLNVS LADTNSLAVVSTQLIMPGQEAG
• Purity: >90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 1.8 mg/mL
• Storage Buffer: PBS, pH7.4, 10% glycerol

Gene Information

• Gene Name: PMEL premelanosome protein [ Homo sapiens (human) ]
• Official Symbol: PMEL
• Synonyms: PMEL; premelanosome protein; SIL, SILV, silver (mouse homolog) like , silver homolog (mouse); melanocyte protein PMEL; D12S53E; gp100; Pmel17; SI; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100; P1; SIL; ME20; P100; SILV; ME20M; ME20-M; PMEL17
• Gene ID: 6490
• mRNA Refseq: NM_001200053
• Protein Refseq: NP_001186982
• MIM: 155550
• UniProt ID: P40967