Recombinant Human PNMT Protein, His-tagged

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Recombinant Human PNMT Protein, His-tagged

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Cat.No. : PNMT-01H
Product Overview : Recombinant Human PNMT Protein (Met1-Leu282) with a C-terminal 6-His tag was expressed in E. coli.
Description : The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.
Source : E. coli
Species : Human
Tag : His
Bio-activity : Measured by its S-Adenosyl-L-Methionine (SAM)-dependent ability to transfer a methyl group to norepinephrine. The specific activity is >2.5 pmol/min/μg.
Molecular Mass : 32 kDa
N-terminal Sequence Analysis : Ser2
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Storage Buffer : Supplied as a 0.2 μm filtered solution in Tris, EDTA, Brij-35 and Glycerol.
Shipping : The product is shipped with polar packs.
Gene Name : PNMT phenylethanolamine N-methyltransferase [ Homo sapiens (human) ]
Official Symbol : PNMT
Synonyms : PNMT; phenylethanolamine N-methyltransferase; PENT; PNMTase; phenylethanolamine N-methyltransferase; noradrenaline N-methyltransferase; phenylethanolamine N-methylase; EC 2.1.1.28
Gene ID : 5409
mRNA Refseq : NM_002686
Protein Refseq : NP_002677
MIM : 171190
UniProt ID : P11086

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