Recombinant Human PNMT Protein, His-tagged
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Cat.No. : | PNMT-01H |
Product Overview : | Recombinant Human PNMT Protein (Met1-Leu282) with a C-terminal 6-His tag was expressed in E. coli. |
Description : | The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Bio-activity : | Measured by its S-Adenosyl-L-Methionine (SAM)-dependent ability to transfer a methyl group to norepinephrine. The specific activity is >2.5 pmol/min/μg. |
Molecular Mass : | 32 kDa |
N-terminal Sequence Analysis : | Ser2 |
Endotoxin : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening. |
Storage Buffer : | Supplied as a 0.2 μm filtered solution in Tris, EDTA, Brij-35 and Glycerol. |
Shipping : | The product is shipped with polar packs. |
Gene Name : | PNMT phenylethanolamine N-methyltransferase [ Homo sapiens (human) ] |
Official Symbol : | PNMT |
Synonyms : | PNMT; phenylethanolamine N-methyltransferase; PENT; PNMTase; phenylethanolamine N-methyltransferase; noradrenaline N-methyltransferase; phenylethanolamine N-methylase; EC 2.1.1.28 |
Gene ID : | 5409 |
mRNA Refseq : | NM_002686 |
Protein Refseq : | NP_002677 |
MIM : | 171190 |
UniProt ID : | P11086 |
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