Recombinant Human POLD2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human POLD2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : POLD2-4964H
Product Overview : POLD2 MS Standard C13 and N15-labeled recombinant protein (NP_006221) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 51.3 kDa
AA Sequence : MFSEQAAQRA HTLLSPPSAN NATFARVPVA TYTNSSQPFR LGERSFSRQY AHIYATRLIQ MRPFLENRAQ QHWGSGVGVK KLCELQPEEK CCVVGTLFKA MPLQPSILRE VSEEHNLLPQ PPRSKYIHPD DELVLEDELQ RIKLKGTIDV SKLVTGTVLA VFGSVRDDGK FLVEDYCFAD LAPQKPAPPL DTDRFVLLVS GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR VILAGNLLSH STQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS DIFRYSSMED HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH VYFCGNTPSF GSKIIRGPED QTVLLVTVPD FSATQTACLV NLRSLACQPI SFSGFGAEDD DLGGLGLGPT RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : POLD2 DNA polymerase delta 2, accessory subunit [ Homo sapiens (human) ]
Official Symbol : POLD2
Synonyms : POLD2; polymerase (DNA directed), delta 2, regulatory subunit 50kDa; polymerase (DNA directed), delta 2, regulatory subunit (50kD); DNA polymerase delta subunit 2; DNA polymerase delta subunit p50;
Gene ID : 5425
mRNA Refseq : NM_006230
Protein Refseq : NP_006221
MIM : 600815
UniProt ID : P49005

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