Recombinant Human POLE2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human POLE2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : POLE2-2637H
Product Overview : POLE2 MS Standard C13 and N15-labeled recombinant protein (NP_002683) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 59.5 kDa
AA Sequence : MAPERLRSRA LSAFKLRGLL LRGEAIKYLT EALQSISELE LEDKLEKIIN AVEKQPLSSN MIERSVVEAA VQECSQSVDE TIEHVFNIIG AFDIPRFVYN SERKKFLPLL MTNHPAPNLF GTPRDKAEMF RERYTILHQR THRHELFTPP VIGSHPDESG SKFQLKTIET LLGSTTKIGD AIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHVNAF GFPPTEPSST TRAYYGNINF FGGPSNTSVK TSAKLKQLEE ENKDAMFVFL SDVWLDQVEV LEKLRIMFAG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKTLADIIC EYPDIHQSSR FVFVPGPEDP GFGSILPRPP LAESITNEFR QRVPFSVFTT NPCRIQYCTQ EITVFREDLV NKMCRNCVRF PSSNLAIPNH FVKTILSQGH LTPLPLYVCP VYWAYDYALR VYPVPDLLVI ADKYDPFTTT NTECLCINPG SFPRSGFSFK VFYPSNKTVE DSKLQGFTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : POLE2 DNA polymerase epsilon 2, accessory subunit [ Homo sapiens (human) ]
Official Symbol : POLE2
Synonyms : POLE2; polymerase (DNA directed), epsilon 2 (p59 subunit); DNA polymerase epsilon subunit 2; DPE2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B;
Gene ID : 5427
mRNA Refseq : NM_002692
Protein Refseq : NP_002683
MIM : 602670
UniProt ID : P56282

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