Recombinant Human Polo-Like Kinase 1, His-tagged

• Cat.No.: PLK1-1085H
• Product Overview: Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLK1 often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLK1 also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 70 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 20 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins; Cell Cycle; Centrosome maturation; DNA Replication; FoxO family signaling; Golgi Cisternae Pericentriolar Stack Reorganization; M Phase; Mitotic G2-G2/M phases; Mitotic M-M/G1 phases; Mitotic Prophase; PLK1 signaling events; Polo-like kinase signaling events in the cell cycle; Progesterone-mediated oocyte maturation

Gene Information

• Gene Name: PLK1 polo-like kinase 1 [ Homo sapiens ]
• Official Symbol: PLK1
• Synonyms: PLK1; polo-like kinase 1; PLK; STPK13; serine/threonine-protein kinase PLK1; PLK-1; polo like kinase; OTTHUMP00000122462; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; cell cycle regulated protein kinase
• Gene ID: 5347
• mRNA Refseq: NM_005030
• Protein Refseq: NP_005021
• MIM: 602098
• UniProt ID: P53350
• Chromosome Location: 16p
• Function: ATP binding; anaphase-promoting complex binding; nucleotide binding; polo kinase kinase activity; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity