Recombinant Human POLR3A Protein (392-632 aa), His-SUMO-tagged

• Cat.No.: POLR3A-730H
• Product Overview: Recombinant Human POLR3A Protein (392-632 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA tplate strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix anates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition . Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as tplate for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 43.4 kDa
• Protein length: 392-632 aa
• AA Sequence: FPEKVNKANINFLRKLVQNGPEVHP GANFIQQRHTQMKRFLKYGNREKMA QELKYGDIVERHLIDGDVVLFNRQP SLHKLSIMAHLARVKPHRTFRFNEC VCTPYNADFDGDEMNLHLPQTEEAK AEALVLMGTKANLVTPRNGEPLIAA IQDFLTGAYLLTLKDTFFDRAKACQ IIASILVGKDEKIKVRLPPPTILKP VTLWTGKQIFSVILRPSDDNPVRAN LRTKGKQYCGKGEDLC
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: POLR3A polymerase (RNA) III (DNA directed) polypeptide A, 155kDa [ Homo sapiens ]
• Official Symbol: POLR3A
• Synonyms: POLR3A; hRPC155; RPC1; RPC155; ADDH; HLD7
• Gene ID: 11128
• mRNA Refseq: NM_007055
• Protein Refseq: NP_008986
• MIM: 614258
• UniProt ID: O14802