Recombinant Human polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa, His-tagged

• Cat.No.: POLR2K-669H
• Product Overview: POLR2K, 1-58aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-58 a.a.
• Description: POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Recombinant human POLR2K protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 9.4 kDa (81aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R
• Purity: >85 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR2K?polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa [?Homo sapiens?(human) ]
• Official Symbol: POLR2K
• Synonyms: POLR2K; RPB12; RPABC4; RPB7.0; hRPB7.0; hsRPB10a; RPB10alpha; ABC10-alpha; polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa; DNA-directed RNA polymerases I, II, and III subunit RPABC4; RNA polymerase II 7.0 kDa subunit; DNA-directed RNA polymerase II subunit K; RNA polymerases I, II, and III subunit ABC4; DNA directed RNA polymerases I, II, and III 7.0 kda polypeptide; NP_005025.1; EC 2.7.7.6
• Gene ID: 5440
• mRNA Refseq: NM_005034
• Protein Refseq: NP_005025
• MIM: 606033
• UniProt ID: P53803
• Chromosome Location: 8q22.2
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat; Cytosolic DNA-sensing pathway; DNA Repair
• Function: DNA binding; contributes_to RNA polymerase I activity; zinc ion binding