Active Recombinant Human PON3 protein(Gly2~Leu354), His-tagged

• Cat.No.: PON3-594H
• Product Overview: Active Recombinant Human PON3 protein(Gly2~Leu354), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.
• Bio-activity: Paraoxonase 3 (PON3) is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein, a function that is believed to slow the initiation and progression of atherosclerosis. Besides, Paraoxonase 1 (PON1) has been identified as an interactor of PON3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON3 and recombinant human PON1. Briefly, PON3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PON1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PON3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PON3 and PON1, and this effect was in a dose dependent manner.
• Molecular Mass: 43.2kDa
• Protein Length: Gly2~Leu354
• Endotoxin: <1.0EU per 1μg (determined by the LAL method)
• Purity: > 98%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PON3 paraoxonase 3 [ Homo sapiens ]
• Official Symbol: PON3
• Synonyms: PON3; paraoxonase 3; serum paraoxonase/lactonase 3; paraoxanase-3
• Gene ID: 5446
• mRNA Refseq: NM_000940
• Protein Refseq: NP_000931
• MIM: 602720
• UniProt ID: Q15166