Recombinant Human POU4F2 protein, GST-tagged

• Cat.No.: POU4F2-21132H
• Product Overview: Recombinant Human POU4F2 protein(1-82 aa), fused with N-terminal GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-82 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 8.0). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 100 mM reduced glutathione (GSH).
• AASequence: MMMMSLNSKQAFSMPHGGSLHVEPKYSALHSTSPGSSAPIAPSASSPSSSSNAGGGGGGGGGGGGGGGRSSSSSSSGSSGGG
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: POU4F2 POU class 4 homeobox 2 [ Homo sapiens ]
• Official Symbol: POU4F2
• Synonyms: POU4F2; POU class 4 homeobox 2; BRN3B, POU domain class 4, transcription factor 2 , POU domain, class 4, transcription factor 2; POU domain, class 4, transcription factor 2; Brn 3b; brain-3B; POU domain protein; Brn3b POU domain transcription factor; POU domain class 4 transcription factor 2; brain-specific homeobox/POU domain protein 3B; BRN3B; BRN3.2; Brn-3b
• Gene ID: 5458
• mRNA Refseq: NM_004575
• Protein Refseq: NP_004566
• MIM: 113725
• UniProt ID: Q12837