Recombinant Human PPP2R1A Protein, Full Length, N-GST tagged

• Cat.No.: PPP2R1A-49H
• Product Overview: Recombinant full-length human PPP2R1A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full Length
• Description: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.
• Molecular Mass: 92 kDa
• Purity: The purity of PPP2R1A was determined to be > 70% by densitometry, approx. MW 92 kDa.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.5 μg/μL
• Storage Buffer: 50mM Tris-HCl pH 7.5, 150mM NaCl,10mM glutathione, 0.1mM EDTA,0.25mMDTT, 0.1mM PMSF, 25% glycerol.
• Shipping: Dry ice
• References: 1. Tang Z, et al: PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation. Dev Cell. 10(5):575-85, 2006.

Gene Information

• Gene Name: PPP2R1A protein phosphatase 2 scaffold subunit Aalpha [ Homo sapiens (human) ]
• Official Symbol: PPP2R1A
• Synonyms: PPP2R1A; protein phosphatase 2 scaffold subunit Aalpha; HJS2; MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha
• Gene ID: 5518
• mRNA Refseq: NM_014225
• Protein Refseq: NP_055040
• MIM: 605983
• UniProt ID: P30153