Recombinant Human PR domain containing 1 cell lysate, transcript variant 1

• Cat.No.: PRDM1-2891HCL
• Product Overview: Recombinant Human PR domain containing 1, transcript variant 1 fused to Flag-tag, was expressed in HEK293T cell.

Specification

• Source: HEK293T cell
• Species: Human
• Molecular Mass: 88.4 kDa
• Components: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
• Storage Instruction: The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: PRDM1 PR domain containing 1, with ZNF domain [ Homo sapiens (human) ]
• Official Symbol: PRDM1
• Synonyms: BLIMP1; PRDI-BF1
• Gene ID: 639
• mRNA Refseq: NM_001198
• Protein Refseq: NP_001189
• UniProt ID: O75626
• Chromosome Location: 6q21
• Pathway: Direct p53 effectors, organism-specific biosystem; NOD pathway, organism-specific biosystem
• Function: RNA polymerase II core promoter proximal region sequence-specific DNA binding; histone deacetylase binding