Recombinant Human PRIM2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PRIM2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PRIM2-5440H
Product Overview : PRIM2 MS Standard C13 and N15-labeled recombinant protein (NP_000938) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 58.8 kDa
AA Sequence : MEFSGRKWRK LRLAGDQRNA SYPHCLQFYL QPPSENISLI EFENLAIDRV KLLKSVENLG VSYVKGTEQY QSKLESELRK LKFSYRENLE DEYEPRRRDH ISHFILRLAY CQSEELRRWF IQQEMDLLRF RFSILPKDKI QDFLKDSQLQ FEAISDEEKT LREQEIVASS PSLSGLKLGF ESIYKIPFAD ALDLFRGRKV YLEDGFAYVP LKDIVAIILN EFRAKLSKAL ALTARSLPAV QSDERLQPLL NHLSHSYTGQ DYSTQGNVGK ISLDQIDLLS TKSFPPCMRQ LHKALRENHH LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIKGKMDPDK FDKGYSYNIR HSFGKEGKRT DYTPFSCLKI ILSNPPSQGD YHGCPFRHSD PELLKQKLQS YKISPGGISQ ILDLVKGTHY QVACQKYFEM IHNVDDCGFS LNHPNQFFCE SQRILNGGKD IKKEPIQPET PQPKPSVQKT KDASSALASL NSSLEMDMEG LEDYFSEDST RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PRIM2 DNA primase subunit 2 [ Homo sapiens (human) ]
Official Symbol : PRIM2
Synonyms : PRIM2; primase, DNA, polypeptide 2 (58kDa); PRIM2A, primase, polypeptide 2A (58kD), primase, polypeptide 2A, 58kDa; DNA primase large subunit; dJ422B11.1.1; DNA primase subunit p58; DNA primase 58 kDa subunit; primase polypeptide 2A, 58kDa; p58; PRIM2A; MGC75142;
Gene ID : 5558
mRNA Refseq : NM_000947
Protein Refseq : NP_000938
MIM : 176636
UniProt ID : P49643
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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