Recombinant Human PRIM2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PRIM2-5440H
• Product Overview: PRIM2 MS Standard C13 and N15-labeled recombinant protein (NP_000938) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 58.8 kDa
• AA Sequence: MEFSGRKWRKLRLAGDQRNASYPHC LQFYLQPPSENISLIEFENLAIDRV KLLKSVENLGVSYVKGTEQYQSKLE SELRKLKFSYRENLEDEYEPRRRDH ISHFILRLAYCQSEELRRWFIQQEM DLLRFRFSILPKDKIQDFLKDSQLQ FEAISDEEKTLREQEIVASSPSLSG LKLGFESIYKIPFADALDLFRGRKV YLEDGFAYVPLKDIVAIILNEFRAK LSKALALTARSLPAVQSDERLQPLL NHLSHSYTGQDYSTQGNVGKISLDQ IDLLSTKSFPPCMRQLHKALRENHH LRHGGRMQYGLFLKGIGLTLEQALQ FWKQEFIKGKMDPDKFDKGYSYNIR HSFGKEGKRTDYTPFSCLKIILSNP PSQGDYHGCPFRHSDPELLKQKLQS YKISPGGISQILDLVKGTHYQVACQ KYFEMIHNVDDCGFSLNHPNQFFCE SQRILNGGKDIKKEPIQPETPQPKP SVQKTKDASSALASLNSSLEMDMEG LEDYFSEDSTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PRIM2 DNA primase subunit 2 [ Homo sapiens (human) ]
• Official Symbol: PRIM2
• Synonyms: PRIM2; primase, DNA, polypeptide 2 (58kDa); PRIM2A, primase, polypeptide 2A (58kD), primase, polypeptide 2A, 58kDa; DNA primase large subunit; dJ422B11.1.1; DNA primase subunit p58; DNA primase 58 kDa subunit; primase polypeptide 2A, 58kDa; p58; PRIM2A; MGC75142
• Gene ID: 5558
• mRNA Refseq: NM_000947
• Protein Refseq: NP_000938
• MIM: 176636
• UniProt ID: P49643